Rust HUBB
Rust HUBB contains 28S DNA sequences from thousands of rust species. Sequences have been verified, and come from vouchered herbarium specimens.
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For details on methods and primers for 28S sequencing, see Aime & McTaggart 2020.
To use this tool, select the Rust HUBB button at the right. Paste your 28S sequence into the box and blast.
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Results will show closest matches in our database, including name, collection number, voucher number, host (family or subfamily, genus and species) and geographic origin, as shown below.
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When interpreting Blast results, in general, conspecificity will be indicated at >99% identity for rusts at this locus. Be sure to compare alignments -- genuine SNPs are more indicative of different species whereas polymorphic sites (Y/C/T) and poly-A/T sites may not be indicative of different species.
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Results should be verified by microscopic examination.
Herbarium codes: PUR = Arthur Fungarium, Purdue University; BPI = US National Fungus Collections, Beltsville, MD; LSUM = Louisiana State University Massey Herbarium, Baton Rouge, LA; BUT = Butler University Herbarium, Indianapolis, IN; BRIP = Plant Pathology Herbarium, Queensland, Australia.
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Citation: Kaishain P, Layug CRK, Anderson M, Berg D, Aime MC. 2024. Introducing Rust HUBB, the rust herbarium-based universal barcode Blast tool. IMA Fungus 15: 3. doi: 10.1186/s43008-023-00132-7
Statistics (Dec. 3, 2023)
Number of sequences: 4019
Number of species: 1150
Number of genera: 125